2. The migration of colloidal particles under the influence of an applied electrical field.
A colloidal particle; such as, a protein molecule, has large numbers of positive and negative radicals which act as if they were on the surface.
Since protein molecules carry electric charges, they will migrate when subjected to an electric field.
3. The migration of dispersed solid, liquid, or gaseous material to one of two electrodes under the influence of an impressed direct-current voltage.2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.
Electrophoretic methods are useful in the analysis of protein mixtures because protein particles move with different velocities depending principally on the number of charges carried by the particles.
3. The movement of charged suspended particles through a liquid medium in response to changes in an electric field.Charged particles of a given substance migrate in a predictable direction and at a characteristic speed.
The pattern of migration can be recorded in bands on an electrophoretogram.
This technique is extensively used to separate and to identify serum proteins and other substances.
Antigens in a gel medium in which the pH is controlled are strongly negatively charged and will migrate rapidly across the electric field toward the anode.
The antibody in such a medium is less negatively charged and will migrate in an opposite or "counter" direction toward the cathode.
If the antigen and antibody are specific for each other, they combine and form a distinct line of precipitation.
This technique is becoming increasingly useful for detecting antigens or antibodies specific for given infectious diseases, diagnosing clinical bacterial infections, and choosing medications to treat the infections.
It is used in two-dimensional immunoelectrophoresis, separation and identification of proteins based on differences in electrical charge and reactivity with antibodies.
Proteins migrate through layered gels of differing pore size and/or pH, forming discontinuous and concentrated disks of individual molecules within the gel layers.
The various types include paper, cascading electrodes, high voltage, gel, etc.
Electrophoresis is the motion of charged particles in a colloid (mixture in which one substance is divided into minute particles, called colloidal particles, and dispersed throughout a second substance) under the influence of an electric field. Particles with a positive charge go to the cathode and negative charge to the anode.
Test substances are driven directly into the medium which contains the antibody, forming rocket-shaped (inverted V) trails of precipitation.
2. Electrophoresis in which antigen migrates from a well through agar gel containing antiserum, forming cone-shaped (rocket) precipitin bands.The area under the cone is used to calculate the amount of antigen.
Zone electrophoresis allows more manipulation of the separated proteins than moving-boundary electrophoresis.