1. The migration of charged colloidal particles or molecules through a solution under the influence of an applied electric field usually provided by immersed electrodes.
2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.

Electrophoretic methods are useful in the analysis of protein mixtures because protein particles move with different velocities depending principally on the number of charges carried by the particles.

3. The movement of charged suspended particles through a liquid medium in response to changes in an electric field.

Charged particles of a given substance migrate in a predictable direction and at a characteristic speed.

The pattern of migration can be recorded in bands on an electrophoretogram.

This technique is extensively used to separate and to identify serum proteins and other substances.