Holding, suturing, or fastening in a position with a process for microscopy that is carried out at low temperatures during surgery to improve the quality of holding body parts together: Cryofixation is often done at very low temperatures and fast coolings are used to prevent formation of ice crystals.

Cooling rates of 10,000 degrees per minute may be used in cryofixations and liquid nitrogen or even liquid helium temperatures are utilized and it is not necessary to include any chemical treatments.