You searched for: “electrophoresis
electrophoresis
1. The movement of charged particles or ions suspended in a solution caused by applying an electric field.
2. The migration of colloidal particles under the influence of an applied electrical field.

A colloidal particle; such as, a protein molecule, has large numbers of positive and negative radicals which act as if they were on the surface.

Since protein molecules carry electric charges, they will migrate when subjected to an electric field.

3. The migration of dispersed solid, liquid, or gaseous material to one of two electrodes under the influence of an impressed direct-current voltage.
This entry is located in the following unit: electro-, electr-, electri- (page 75)
electrophoresis, cataphoresis
1. The migration of charged colloidal particles or molecules through a solution under the influence of an applied electric field usually provided by immersed electrodes.
2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.

Electrophoretic methods are useful in the analysis of protein mixtures because protein particles move with different velocities depending principally on the number of charges carried by the particles.

3. The movement of charged suspended particles through a liquid medium in response to changes in an electric field.

Charged particles of a given substance migrate in a predictable direction and at a characteristic speed.

The pattern of migration can be recorded in bands on an electrophoretogram.

This technique is extensively used to separate and to identify serum proteins and other substances.

Word Entries containing the term: “electrophoresis
counterimmunoelectrophoresis, CIE, counter electrophoresis, countercurrent electrophoresis, counter migration electrophoresis
A laboratory technique in which an electric current is used to accelerate the migration of an antibody and an antigen through a buffered diffusion medium.

Antigens in a gel medium in which the pH is controlled are strongly negatively charged and will migrate rapidly across the electric field toward the anode.

The antibody in such a medium is less negatively charged and will migrate in an opposite or "counter" direction toward the cathode.

If the antigen and antibody are specific for each other, they combine and form a distinct line of precipitation.

This technique is becoming increasingly useful for detecting antigens or antibodies specific for given infectious diseases, diagnosing clinical bacterial infections, and choosing medications to treat the infections.

This entry is located in the following unit: electro-, electr-, electri- (page 3)
crossed electrophoresis (s) (noun), crossed electrophoreses (pl)
A technique for electrophoretic separation (science of objects moving in a fluid when an electric charge is applied) of mixed proteins in which two successive currents are passed through the support medium in directions at right angles to each other.

It is used in two-dimensional immunoelectrophoresis, separation and identification of proteins based on differences in electrical charge and reactivity with antibodies.

This entry is located in the following units: cruci-, crux (page 1) electro-, electr-, electri- (page 3)
disk electrophoresis
An electrophoretic technique (migration of electrically charged particles) which separates proteins by their physical interaction with the supporting medium as well as by electrophoretic mobility.

Proteins migrate through layered gels of differing pore size and/or pH, forming discontinuous and concentrated disks of individual molecules within the gel layers.

This entry is located in the following unit: electro-, electr-, electri- (page 4)
electrophoresis apparatus
A device for causing migration of charged particles (ions) in solution in an electric field.

The various types include paper, cascading electrodes, high voltage, gel, etc.

This entry is located in the following unit: electro-, electr-, electri- (page 75)
electrophoresis scanner
An instrument for reading bands on paper strips or gel, for the purpose of measuring particle movements because of electrophoresis.
This entry is located in the following unit: electro-, electr-, electri- (page 76)
gel electrophoresis
An electrophoretic technique in which the charged material migrates through an agar gel (gelatinous product extracted from certain red algae) rather than on a fluid-impregnated solid strip.
This entry is located in the following unit: electro-, electr-, electri- (page 92)
high-voltage electrophoresis
Electrophoresis, often using paper as a support to the solution, in which a high potential, often a few kilovolts, is used.

Electrophoresis is the motion of charged particles in a colloid (mixture in which one substance is divided into minute particles, called colloidal particles, and dispersed throughout a second substance) under the influence of an electric field. Particles with a positive charge go to the cathode and negative charge to the anode.

This entry is located in the following unit: electro-, electr-, electri- (page 93)
isoenzyme electrophoresis
An electrophoretic technique (movement of electricity charged particles) used to separate the different forms of a single enzyme produced by cells of different origin.
This entry is located in the following unit: electro-, electr-, electri- (page 94)
lipoprotein electrophoresis
A technique for semiquantitative analysis of plasma lipoproteins by electrophoretic separation followed by staining and quantification of the separated lipoprotein bands.
This entry is located in the following unit: electro-, electr-, electri- (page 94)
moving boundary electrophoresis
An electrophoretic technique (movement of electrically charged particles in a fluid or gel) in which an optical system is used to observe and to measure the electrophoretic movement of the distinct boundaries in a suspension.
This entry is located in the following unit: electro-, electr-, electri- (page 95)
paper electrophoresis
An electrophoretic technique (movement of electricity charged particles in a fluid) in which the charged materials migrate on a paper strip impregnated with an electrolyte solution through which the electric current passes.
This entry is located in the following unit: electro-, electr-, electri- (page 96)
rocket electrophoresis
1. A variant of crossed electrophoresis in which the medium contains only one antibody.

Test substances are driven directly into the medium which contains the antibody, forming rocket-shaped (inverted V) trails of precipitation.

2. Electrophoresis in which antigen migrates from a well through agar gel containing antiserum, forming cone-shaped (rocket) precipitin bands.

The area under the cone is used to calculate the amount of antigen.

This entry is located in the following unit: electro-, electr-, electri- (page 99)
thin-layer electrophoresis
Zone electrophoresis in which the supporting medium is applied in a thin layer to a glass or plastic strip.
This entry is located in the following unit: electro-, electr-, electri- (page 101)
zone electrophoresis
The electrophoretic separation of migrating molecules in a conducting medium which is immobilized onto an inert supporting medium such as paper, cellulose acetate, or agar gel.

Zone electrophoresis allows more manipulation of the separated proteins than moving-boundary electrophoresis.

This entry is located in the following unit: electro-, electr-, electri- (page 102)